The Frenchman did not train with the rest of his team-mates in the final session before Sunday’s Liga derby, in which he will not feature.
Benzema had been in a race against time recover from the knock ahead of the Liga clash, however he did not train with his tea- mates in the final session before the game.
The France international is now doubtful for his country’s fixtures during the international break, and may stay in Madrid ahead of the final months of the season.
What has happened to Benzema?
The striker has an injury to his left calf, which forced him to be subsituted after suffering it during the 3-0 Liga win at Mallorca on Monday.
It is not a serious injury and Benzema will be available after the two-week international break, however he has been ruled out for the huge clash with rivals Barcelona.
Madrid host Barca on Sunday, aiming to extend their 10 point lead over second-placed Sevilla in La Liga and their 15-point advantage over the Blaugrana in third.
Will Benzema miss crucial games?
Crucially for Madrid, however, Benzema is expected to be back ahead of April 6, when they face Chelsea in the first leg of their Champions League quarter-final clash.
Benzema was central to Madrid reaching the last eight, with his hat-trick turning around the second leg of their last 16 meeting with PSG, as the Spanish side won 3-2 on aggregate.